The rate of the decomposition of hydrogen peroxide is changed by using a catalyst. This is called the maximum velocity of the reaction or Vmax. This means there will be twice as many successful collisions. November 7, at Weigh the yeast directly into the conical flask, not a Petri dish, so you do not have to worry about losing yeast mass when transferring it from the Petri dish to the conical flask. Enzymes are classified into several categories, such as hydrolytic, oxidising, and educing.
Catalase is an enzyme found in living cells which catalyzes the decomposition of hydrogen peroxide into water and oxygen. Enter a valid email address. All the active sites are being used so any extra Hydrogen Peroxide molecules will have to wait until an active site becomes available. ComScore is a media measurement and analytics company providing marketing data and analytics to enterprises, media and advertising agencies, and publishers. The average results are all written down to one decimal place because although the stopwatch gives results to two decimal places it is impossible to get accurate times to two decimal places due to the fact that our reaction times are not fast enough to stop the stopwatch precisely. However, there are some factors that I must take into consideration.
Google provides ad serving technology and runs an ad network. This meant that less gas evolved with time, so I could effectively time and measure the volume of oxygen produced. Biology Catalase Coursework – homeconnectionuk. The rate of reaction can be measured by measuring the volume of oxygen produced cougsework a period of time.
Biology coursework comparison between plant and animal sources of catalase in hydrolysing hydrogen peroxide. Obtained 20 test tubes and 4 test tube rack 2. We extract catalase from grinded potatoes and add increasing amount of catalase to hydrogen peroxide in separate test tubes.
Effect of Substrate Concentration on the Rate of Activity of Catalase
I predict that the higher the concentration andd celery extract I use, the peeroxide the rate of breaking down hydrogen peroxide I will get.
I would expect the rate to increase two times if the Hydrogen Peroxide concentration is increased two times because there are twice as many substrate molecules which can join onto the enzymes active sites. Disclaimer This is a real A-level school project and as such is intended for educational or research purposes only.
May 14, at 4: October 8, at 6: Control Temperature With a Water Bath In the main procedure, I will control the temperature with a water bath in order to create a constant external temperature and dissipate the heat energy. It is very important to accurately measure the amounts of Hydrogen Peroxide, Yeast and water to ensure a fair test.
I then worked hydrogsn the rates of the reactions with the equation. How to cite this page Choose cite format: The plotted results on the graph produce a straight line of best fit to begin with which then goes into a curve of steadily decreasing gradient.
Once the amount of substrate molecules peroxids exceeds the number of active sites available then ccatalase rate of reaction will no longer go up.
The final hjdrogen of oxygen produced also decreased as the concentration decreased. Draw a new graph. As the concentration increasedthe amount of oxygen decrease?
You have been warned. As enzyme work best at a certain temperature, this is known as optimum temperature. Therefore, reaction is slow. I was wondering how i could reference this page in APA format. This is used to identify particular browsers or devices when the access the service, and is used for security reasons. Hydrogen peroxide is also a dangerous, very catallase by-product of metabolism, and it is essential that it is broken down quickly so it does not cause damage to cells.
Final Volume of Oxygen in cm cubed. So the indepent variable is the yeast and the indepent variable should be the x-axis!
size gcse coursework biology potato catalase hydrogen peroxide
Because the volume of oxygen dissolved in the reaction is not constant for all reactions, and less oxygen is dissolved in water at higher temperatures, this would have affected my results. As globular protein, enzymes have a specific three-dimensional shape which is determined by their sequence of amino acids. This will minimise the effect of temperature on the results of the experiment. I will investigate if this is true for this reaction. Catalase is an enzyme which is found courseworl most living organisms.
Results Below is a table of the results I collected, including all the results which I had to repeat.
Experimeny Us on the Move. Hopefully, with the new and repeated results, I will be able to analyse my results further and therefore evaluate them with more evidence than I had previously.